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precedente: S1
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livello superiore: S1
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seguente: S1.2
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S1.1 - Molecular ecotoxicology: the systems biology approach reveals stress
response pathways in the social amoeba Dictyostelium discoideum
challenged with low mercury concentrations
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Viarengo Aldo (1)*
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Dipart. di Scienze dell'Ambiente e della Vita, Università del
Piemonte Orientale, Via Bellini 25G, 15100 Alessandria, Italia |
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viarengo@unipmn.it |
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A combination of genomic/proteomic and cellular techniques was used to assess
global effects of Hg exposure on the social amoeba Dictyostelium
discoideum. Our data demonstrated that sublethal concentrations of Hg affect
important physiological parameters such as cytosolic free calcium
concentration, lysosomal membrane stability and endocytosis, without altering
cell replication and survival. Molecular mechanisms behind Hg-response were
studied by DNA microarray transcriptomic analysis. The co-activation of genes
coding for GSH conjugating enzymes and transmembrane extrusion proteins (ABC)
are likely to represent the first defence line against Hg toxicity. Other up-regulated genes were involved in toxicity resistance, detoxification,
energy metabolism and specific redox processes. Moreover, extracts of control
and treated cells were analysed by two-dimensional electrophoresis. A
reference map containing some 900 proteins was obtained. Identification of
excised spots was performed by MALDI-TOF peptide mass fingerprinting, and the
identity of some 150 proteins was revealed. Fifteen proteins involved mainly
in detoxification and signal transduction pathways as well as a hypothetical
protein possibly related to energy metabolism showed a dramatic increase in
Hg-treated cells. In conclusion, systems biology can represent a potent tool
to characterize the molecular fingerprinting and the adaptative responses to
hazardous pollutants present in the environment.
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consulta l' Indice analitico (alfabetico per autore) |
sfoglia l' Indice delle sessioni del Congresso |
a cura di Comoglio, Comino, e Bona
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