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S5.1 - Metallothionein in Perna perna: Potential biomarker for monitoring heavy metal pollution in Guanabara Bay, Rio de Janeiro, Brazil

Mao Andrea (1)*, Bottos Daniela (1), Manca Daniela (1), Manente Sabrina (1), Batalha Fernando (2), Verçosa Carvalheira Lucia (3), Perin Guido (1)

(1) Dipart. di Scienze Ambientali, Università Ca' Foscari di Venezia, Calle Larga S. Marta 2137 - Dorsoduro, 30123 Venezia, Italia
(2) FEEMA (Fundação Estadual de Engenharia do Meio Ambiente), Avenida Nossa Senhora de Copacabana, Rio de Janeiro, Brasil
(3) UERJ - Universidade do Estado do Rio de Janeiro, Departamento de Oceanografia, Rua São Francisco Xavier 524, Rio de Janeiro, Brasil
* mao@unive.it

Guanabara Bay is one of the largest coastal bays in Brazil; this bay measures 31 km from south to north and has a surface area of about 400 km2 of estuarine waters and is important for southeast Brazilian coast fisheries ecology. It is an eutrophic, polluted system, impacted by largely untreated domestic runoff from the 8 million human inhabitants of its basin. Metallothioneins (MTs) induction as a response to metal exposure is well documented, and thus MTs have been proposed as biomarkers of trace metal pollution in numerous species from different zoological groups. In the present study the mussel Perna perna was collected in Guanabara Bay during December 2004. More precisely, sampling (8 sites) was realized in the south part of Bay; determination of MTs has been done in the mussel digestive gland, estimating sulphydrylic groups residue content using Ellman's reagent. The response of mussels exposed to metals in well-contrasted sites in terms of metal availability was investigated, but avoiding intersite significant differences in natural parameters such as temperature and salinity. In fact the relevance of the use of MTs concentrations as biomarkers of metal pollution depends on the relative importance of natural factors and contamination factors in governing the concentration of this protein in organisms or in peculiar organs.


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