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S2.4 - Monogenoids in reef associated fish species from the Red Sea, Ras Mohammed National Park, Egypt: preliminary results based on a novel laser-scanning confocal fluorescence microscopy technique

Galli Paolo (1)*, Villa Anna Maria (1), Strona Giovanni (1), Benzoni Francesca (1), Stefani Fabrizio (1), Doglia Silvia Maria (1), Kritsky Delane (2)

(1) Dipart. di Biotecnologie e Bioscienze, Università degli Studi di Milano Bicocca, Piazza della Scienza 2, 20126 Milano, Italia
(2) Dept. of Health and Nutrition Sciences, Idaho State University, Campus Box 8090, Idaho State University, Pocatello Idaho, USA
* paolo.galli@unimib.it

A parasitological survey of the monogenoids of sixteen fish species (Apogon cyanosoma, Crenimugil crenilabis, Epinephelus fasciatus, Gerres oyena, Lethrinus nebulosus, Lethrinus variegatusMulloidichthys flavolineatus, Mulloidichthys vanicolensis, Siganus rivulatus, Lethrinus borbonicus, Plectorhinchus schotaf, Tylosurus choram, Sphyraena flavicauda) belonging to the ichthyofauna of the Ras Mohammed National Park, Egypt, was carried out during May 2003. The present study aims to contribute to the knowledge of the fish parasite fauna from a largely unstudied area. The monogenoids from the Park were studied with a novel technique. Laser scanning confocal microscopy images were collected using the Leica TCS SP2 confocal microscope equipped with an inverted Leica DMIRE2 microscope and a PL APO 63x oil immersion objective. An Ar laser was used for fluorescence excitation at 515 nm; fluorescence was detected between 525 and 730 nm. Images were collected at 8-bit with 1,024x1,024 pixels per frame. The monogenoids from the examined fish consisted of 19 species subdivisible into 6 known species, 7 known genera, 4 genera ascribable to Haliotrema sensu lato and 4 to new genera. Our observations resulted in an average number of 1,5 monogenean species per fish species, leading us to a provisional estimate of roughly 1,800 species for the whole Red Sea ichthyofauna. Further analyses are needed to improve our knowledge of monogenoids diversity.


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