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precedente: S1.7
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livello superiore: S1
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seguente: S1.9
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S1.8 - Cellular responses in mussels (Mytilus galloprovincialis)
transplanted to the estuary of Cecina river
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Del Barga Ilaria (1)*,
Frenzilli Giada (1),
Falleni Alessandra (1),
Scarcelli Vittoria (1),
Lucchesi Paolo (1),
Ghezzani Claudio (1),
Nigro Marco (1)
| (1) |
Dipart. di Morfologia Umana e Biologia Applicata, Università
degli Studi di Pisa, Via Volta 4, 56126 Pisa, Italia |
| * |
ilariadelbarga@libero.it |
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Although its modest extension, the basin of Cecina has been indicated as
pilot basin within the Water Frame Directive (2000/60 EC). It flows across
Tuscany and is impacted by several human activities including mining and
tanning industry, geothermal power plants and agriculture. Chemical analyses
showed that sediments and biota are contaminated by heavy metals and
pesticides. Cecina river enters the Central Tyrrenian Sea (South Tuscany), an
area with an high touristic importance.
Cellular responses were investigated in order to evaluate possible early
warning signals.
Mussels from a commercial farm were caged for one month in a reference site
and into the estuary of the river. Lysosomal membrane instability was detected
in haemocytes and lipofuscin accumulation was observed in cryopreserved
digestive glands. Lysosomal enlargements were also quantified by electron
microscopy. Genotoxic effects were evaluated by Comet assay and Micronucleus
assay in gill cells.
Organisms deployed at the impacted site showed a lower (p<0.01) lysosomal
membrane stability and a larger (p<0.05) diameter in comparison with controls.
They also displayed lower (p<0.01) levels of DNA integrity, accompanied by
higher (p<0.05) frequencies of micronucleated cells, indicating the presence
of environmental stress in the area under investigation.
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consulta l' Indice analitico (alfabetico per autore) |
sfoglia l' Indice delle sessioni del Congresso |
a cura di Comoglio, Comino, e Bona
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