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precedente: S1.6
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livello superiore: S1
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seguente: S1.8
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S1.7 - Dolphins in test tubes: new-tool to investigate toxicological hazard due
to endocrine disruptor organochlorines and emerging contaminants in
mediterranean cetaceans
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Fossi Maria Cristina (1)*,
Marsili Letizia (1),
Casini Silvia (1),
Bucalossi Daniela (1)
| (1) |
Dipart. di Scienze Ambientali 'G. Sarfatti', Università degli
Studi di Siena, Via Mattioli 4, 53100 Siena, Italia |
| * |
fossi@unisi.it |
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Mediterranean cetaceans, particularly odontocetes, accumulate high
concentrations of organochlorine contaminants (OCs) and are therefore exposed
to high toxicological risk. Some OCs are known to be endocrine disrupting
compounds (EDCs). The possibility that certain Mediterranean cetaceans Stenella coeruleoalba, Delphinus delphis, Tursiops truncatus and Balaenoptera physalus are subject to toxicological risk due to
organochlorines and emerging contaminants, such as polybrominated diphenyl
ethers (PBDEs) with endocrine disrupting capacity, was investigated using
non-lethal 'diagnostic' and 'prognostic' methods. Benzo(a)pyrene
monooxygenase (CYP1A1) activity in skin biopsies was used as a 'diagnostic'
indicator of exposure to organochlorines in odontocetes and mysticetes and in
different populations of S. coeruleoalba. Marked differences in levels
of OCs and CYP1A1 activity were found between fin whales and odontocetes.
Organochlorine levels and CYP1A1 activity were significantly higher in the S.
coeruleoalba population of the Mediterranean Whale Sanctuary than in those of
two other study areas, suggesting that cetaceans are exposed to high risk in
this protected area. As a new 'prognostic' tool we explored interspecies and
gender susceptibility to OC-EDCs and PBDEs using qualitative and
semi-quantitative evaluation of target proteins, such as CYP450 1A1 and CYP450
2B in cultured cetacean fibroblasts, by western blot, immunofluorescence
technique and PCR real time.
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consulta l' Indice analitico (alfabetico per autore) |
sfoglia l' Indice delle sessioni del Congresso |
a cura di Comoglio, Comino, e Bona
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