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precedente: S1.5
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livello superiore: S1
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seguente: S1.7
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S1.6 - Application of the Comet assay to Dreissena polymorpha haemocytes
for genotoxicity monitoring of Lake Maggiore
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Riva Consuelo (1)*,
Binelli Andrea (1),
Provini Alfredo (1)
| (1) |
Dipart. di Biologia, Sez. di Ecologia, Università degli Studi
di Milano, Via Celoria 26, 20133 Milano, Italia |
| * |
consuelo.riva@unimi.it |
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The exposure of an organism to genotoxic chemicals may induce alteration in
DNA, such as base modifications, strand breaks and cross-linkages. The
assessment of these modifications can be used as genetic biomarker of exposure
to damage-causing agents. Single cell gel electrophoresis method, commonly
known as Comet assay, has proved to be a rapid, versatile and easily utilized
tool for collecting data on DNA damage (single-, double-strand breaks, alkali
labile sites or DNA-DNA and DNA-protein crosslinks) in individual cells.
Suitable organisms can be used in combination with this test as bioindicators
for contamination of the environments with genotoxins, but despite increased
interest, few studies has been surprisingly done using the Comet assay on
freshwater species and almost none on freshwater invertebrates.
The aim of this study was to use the Comet assay on haemocytes of the
freshwater mussel Dreissena polymorpha to assess the environmental
quality of Lake Maggiore, a well-known heavy polluted freshwater ecosystem.
Specimens collected in sites with different levels of contamination
highlighted significant different degree of DNA damage in comparison with
controls maintained at laboratory conditions. This research showed that the
use of haemocytes from D. polymorpha in Comet assay should be a
sensitive, even if non specific tool in assessing genotoxicity in the
freshwater environments.
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consulta l' Indice analitico (alfabetico per autore) |
sfoglia l' Indice delle sessioni del Congresso |
a cura di Comoglio, Comino, e Bona
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