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S1.5 - New Biondicators for ecotoxicological assessment of Mediterranean off-shore extraction: barnacles (Balanus trigonus and Balanus perforatus)

Mori Gabriele (1)*, Ferraro Maria (2), Fossi Maria Cristina (1), Casini Silvia (1), Marsili Letizia (1), Stefanini Giacomo (1), Porcelloni Serena (1), Caliani Ilaria (1), Bianchi Nicola (1), Maltese Silvia (1), Alberti di Catenaia Cesare (2)

(1) Dipart. di Scienze Ambientali 'G.Sarfatti', Università degli Studi di Siena, Via Mattioli 4, 53100 Siena, Italia
(2) Eni Division Exploration and Production, Via Emilia 1, 20097 S. Donato Milanese (MI), Italia
* mori22@unisi.it

The aim of this study is to validate a methodology based on biomarker responses in the barnacles (Balanus trigonus and Balanus perforatus) to assess ecotoxicological effects associated with off-shore extraction. This study is a part of the Biomare project, between the Biomarkers Laboratory (Department of Environmental Science, Siena University) and ENI Exploration and Production Division. During laboratory phase (I) biomarker responses in barnacles exposed to gasoline (10, 100, 1,000 ppm) and produced waters were investigated. In phase (II) biomarkers were validated during a biomonitoring program in the Mediterranean Sea. The selected platform was 'Cervia B' in the northern Adriatic; the 'Paguro' platform, sunk in 1965, was chosen as a comparison site. Several biomarkers (MFO activities (BPMO, Reductases), AChE, antioxidant enzymes, porphyrins) and contaminants (PAHs and trace metals) were measured. Laboratory and field studies showed high sensitivity of response in both species for all biomarkers tested, particularly MFO and esterases, and high bioaccumulation capacity. In conclusion, this study provided innovative results for the use of the selected bioindicators and for the responses generated after chronic and acute exposure to petroleum derivatives. These results suggest that barnacles could be used in future biomonitoring programs to evaluate both the effects of petroleum derived contamination, and the level of contamination itself.


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